The present study aimed to investigate the prevalence of Campylobacter (C) and its clinicopathological changes in broilers in Sharkia province, About 50 diarrheic broilers cloacal swabs were collected for bacteriological examination. Out 50 exami-ned swabs 12 (24%) were positive for Campylobacter [C. coli 4 and C. jejuni 8]. Isolated Campylobacter was sensitive to neomycin and genta-ycin. About 45 healthy one day-old broilers were received 5 mg pefloxacin/kg bwt for 5 days for proved that broilers free from bacterial infections. At 14th day of age broilers were divided into 3 groups (15/ each). 1st group healthy broilers non treated (control), broilers in 2nd and 3rd groups were infected with C. jejuni. 2nd group infected non treated and 3rd group infected, treated with 15 mg neomycin/kg B.wt. in drinking water for 5 days. At 1st, 7th and14th day post treatment cloacal swabs were collected for re-isolation C. jejuni beside blood samples were collected for hematochemical study. Infected broilers showed of food, depression, ruffled feather, diarrhea and mortality rate 40% beside significant decrease in body performance, total protein albumin, globulin coupled with insignificant elevation in RBCs, HB, PCV%, significant elevation in WBCs, AST, ALT, ALP, urea and creatinine. Treatment infected broi-lers by neomycin lead to disappear clinical sing, reduced mortality and improved hematobiochemical parameters. It could be concluded that Campylobact-er infection induces reversible adverse effect on body performance and hematobioc- al parameters. Neomycin is highly curative against campylobacters