The objective of the present study was to evaluate clinico-biochemical changes occurred in bacterial diarrhea in Egyptian Buffalo calves less than three months old. The study was carried out on 50 buffalo calves in El-Galaa Military farm that located in Ismailia Governorate, 40 diarrheic calves aged 55-75 days with an average body weight (35±2.5) kg were detected and 10 apparently healthy calves were kept as a control group to investigate the clinico-biochemical profiles. E. coli mixed or co-infection of Acinetobacterbaumannii/Iwoffiiss species with Klebsiella and pseudomonas species were isolated from the diarrheic calves. Diarrheic calves show signs of anorexia, weakness, dullness, staggering movement, weight loss, pale mucous membranes with sunken eyes and dehydration. Analysis of clinical and biochemical profile of the diarrheic calves revealed significant increase in body temperature, respiratory rate, skin fold test, serum potassium, chloride, urea, creatinine, ALT, AST, GGT, triacylglycerols, cortisol, CRP and CPK withsignificant decrease in pulse rate, serum sodium, glucose and total protein concentrations.It could be concluded that, bacterial diarrhea has a severe negative impact on the clinical and biochemical profile of the affected buffalo calves.