Many bacterial detection rapid methods developed including nucleic acid based analysis which considered the most precise, sensitive, and famous method of detection. This study aimed to investigate the bacterial hygienic quality of some chicken meat and giblet with special concern of molecular detection of some virulence factors associated with some isolated food poisoning bacteria. E. coli, Salmonella, S. aureus, and Y. enterocolitica strains were isolated from commercial and home-reared chicken meat and giblet in Menoufiya Governorate, Egypt. Accurately, stx1, stx2, eaeA, and hylA genes were detected in 45.4, 63.6, 18.1, and 27.2% of the isolated E. coli strains, respectively. invA, hilA, and fimH genes were detected in 100, 71.4, and 85.7% of the examined Salmonella isolates, respectively. Regarding to the examined Y. enterocolitica isolates, Inv gene was detected lonely in 25%, while it was mixed with ystA gene in 75% of the examined isolates. Detection of enterotoxigenic Staph. aureus genes revealed detection of staphylococcal enterotoxins genes types SEA, and SEB genes in 20, and 10%; moreover, mixed SEA+SED, SEB+SEC producing genes were detected in 10% for each, respectively. The present results proved that PCR assay is helpful, rapid and accurate detection method. Strict hygienic measures during slaughtering and handling of chicken meat and giblet must be followed.