The present study aims to detect the prevalence and molecular characterization of Bovine viral diarrhea, Rota and corona viral diseases as major causes of diarrhea that mainly leads to neonatal deaths among neonatal calves in some governorates in Egypt (Kafr El Sheikh, El Menofyia, Sharkia, Bahaira and El-Gharbia). The infection rate of the three examined viral diseases uses showed non-significant difference between sex and age of cow calves, and the infection more common on female younger calves. Also the infection rate in female young buffalo calves. Selective positive samples undergo Sequencing and compared with other strains in GenBank. The results showed that the Egyptian BVD strain is closely related to BVD strain from Argentina (DQ088995) and Colombia (MH198305), while Bovine Rota (BRV) showed high similarity with Bovine Rota strain of South Korea and Turkey but Bovine Corona virus (BCoV) was closed to Bovine Corona viruses (BCoV) strain isolated from France .