This work was carried out to evaluate the efficacy of locally prepared concentrated combined inactivated vaccines comprising BVD, BOHV-1, PI-3 and BRS viruses adjuvanted with aluminum hydroxide gel or montanide oil ISA206 under field condition.
The prepared vaccines adjuvanted aluminum hydroxide gel or montanide oil ISA206 were administered to 6-9-month-old seronegative calves through intramuscular (IM) route at different doses 2.5ml and 3ml respectively. The calves were boostered after two weeks and sera were collected two weeks after vaccination then monthly for 6^th month postvaccination. The induced antibodies were tested using Serum Neutralization Test (SNT). Our results revealed that, the concentrated vaccine has a significant effect on serum antibody levels by the 2^nd wpv till 6 mpv. Moreover, the concentrated vaccine adjuvanted by montanide oil showed prolongation of antibody in comparison to the adjuvanted with aluminum hydroxide gel.