The aim of this work is to investigate the presence of Campylobacter species. in chicken, cattle, some animal products (raw milk, kariesh cheese and yoghurt) in addition to human beings and to confirm its presence by molecular methods. 315 samples were collected from chicken, 150 samples from cattle carcasses, 122 samples from animal products and 128 human stool swabs (hospitalized patients suffering from diarrhea admitted to Toukh Central Hospital). All samples were collected from Toukh city, Kalyoubia governorate, Egypt.  Campylobacter spp. were isolated from chicken samples at percentage of 27.6% from intestinal contents swabs , 27.14% from  liver samples, 7.14% from breast muscles and 14.2% from thigh muscles. In cattle carcasses Campylobacter spp. were isolated with a percentage of 6% from intestinal contents swabs, 6% from liver samples and muscles (2%).Moreover the isolation rate of Campylobacter spp < /em>. from animal product samples was7.4%.11.53% from raw milk, 7.5% from kariesh cheese and 0% from yoghurt. In human Campylobacter spp < /em>. were isolated from 26 stool swabs (20.3%).  Suspected strains were selected according to their biochemical testing and subjected to molecular investigations by using specific primers (mapA gene specific for C.jejuni & ceuE gene specific for C.coli) . Amplification of mapA gene of  C.jejuni & ceuE gene for C.coli isolated from the above mentioned samples have shown identical fingerprints with human isolates at 589 and 462bp for C.jejuni and C.coli respectively ensuring the public health importance of the isolates. From the results of the current study, it could be concluded that Chicken, cattle and animal products are possible sources of human Campylobacter infections.