This study aimed to evaluate the role of estimated levels of miRNA 21 and 126 for differentiation between asymptomatic and symptomatic CHB patients.All subjects enrolled of this study included the small sample size, expensivet echnique and also the limited diversity ofthe patient's blood bank at Benha and University hospitals for blood donation attending Benha University Specialized Hospital,  Such   limitations  can   be over come in further researches.The study included 75 patients diagnosed as CHC (Group I) and 25 healthy controls (Group II) . Group (I) was further subdivided according to the last HBsAg RT- PCR into two groups: Group IA including 25 patients with HBV- PCR positive Group IB including 25 patients with HBV- PCR negative.  All individuals included were subjected to measurement of AST, ALT, ALB, AFP and HBsAg load .the study showed high statistical significant difference.
 (P>0.001) between CHC patients and control group regarding ALT, ASTand AFP being higher in the former group. On the other hand,serum level (P>0.001) betweenALB showed a high statistical significant difference.Both groups being decreased in CHC patients.(P>0.005) of serum   similarly,there was statistical significant increase AST level and decrease of serum AFP in group IA when compared to group IB.Similary, statistical significant difference of both miR 21 and miR126  expression  levels in the peripheral blood  being  high in group IA when compared to group IB (P > 0.001). On the other hand, there was no significant difference between group IB and group II as regard peripheral blood miR- 21 and miR- 126 expressions.Regarding the correction between miR- 21 and miR- 126 expression levels in peripheral blood and each ALT and AST, the study showed a significant positive  correlation. On the other hand , there was statistical negative correction with AFP  level group I and group IA .  in conculusion in study miRNA 21 is more senstivity miRNA 126 in diagnosis hepatitis Bvirus ifection.