β-glucans are heterogeneous group of natural polysaccharides mostly investigated for their immunological effects. The objective of this study was to evaluate the efficiency of irradiated β-glucan on N-nitrosodiethylamine induced hepatocarcinogenesis in rats.β-glucan powder was exposed to 50 KGY of gamma radiation for experimental study, healthy male rats were divided in five groups. Group 1: did not receive any treatment. Group 2: hepatic cancer group rats administrated with DEN orally at a dose of (20mg/kg b.wt/day) for six weeks. Group 3: Irradiated β-glucan group rats were orally administrated with Iβ- glucan for six weeks at a dose of 65 mg/kg body weight/day. Group 4: Protective group rats were administrated Iβ-glucan daily as in group 3 for two weeks they induced with DEN concomitant with Iβ-glucan for six weeks. Group 5: Therapeutic group rats were induced daily with DEN then administrated with Iβ-glucan for six weeks. DEN induced liver damage as evidence by a marked increase in serum indices of liver marker enzymes (Alt, Ast and GGT), tumor markers (alpha fetoprotein) and proinflammatory cytokines(interleukin-6) when compared with the normal control group.  However, administration of irradiated β-glucan, in liver cancer induced rats displayed improvement in all measured parameters. In conclusion Iβ-glucan  considered as  a preventive and a therapeutic towards hepatocarcinogenesis via enhancing immune system in particular CD4, CD8 count and anti-inflammatory effect.