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96293

In vivo cloning of plasmids in E. coli is a simpler and faster method for cloning

Article

Last updated: 24 Dec 2024

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Subjects

-

Tags

Anatomy and embryology

Abstract

DNA assembly sequences into plasmids is one of the most important basic technologies for bioscience research and metabolic engineering. There are many of molecular cloning techniques have been developed and these techniques that need specialized expensive reagents or laborious experimental procedure. For that reason, a significant amount of effort has been dedicated to developing better DNA assembly methods with higher efficiency and fidelity as well as simpler and faster protocols. Here, we compared between conventional, in vivo and in vitro DNA assembly methods and their recent applications, we also highlight the optimum protocol for in vivo cloning of DNA assembly methods. The present study concluded that vector construction can be carried out simply by simply placing a DNA fragment having a homologous sequence and directly transformed into E. coli and this method gives great help in improving efficiency of molecular biological research.  

DOI

10.21608/bvmj.2018.96293

Keywords

in vivo cloning, PCR, conventional cloning, transformation and invitro cloning

Authors

First Name

Ahmed

Last Name

Osama

MiddleName

-

Affiliation

Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Benha University

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Attia

MiddleName

-

Affiliation

Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Benha University

Email

mohamed.mitwalli@fvtm.bu.edu.eg

City

-

Orcid

-

First Name

Hatem

Last Name

Bahgat

MiddleName

-

Affiliation

Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Benha University

Email

amanyabbass65@gmail.com

City

-

Orcid

0000-0001-7061-8496

First Name

Ahmed

Last Name

Kassab

MiddleName

-

Affiliation

Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Benha University

Email

ahmed.kassab@fvtm.bu.edu.eg

City

-

Orcid

-

First Name

Masatake

Last Name

Osawa

MiddleName

-

Affiliation

Laboratory of Molecular design and Synthesis, Department of Regenerative Medicine and Applied Medical Science, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan.

Email

masaosaw@gifu-u.ac.jp

City

-

Orcid

-

First Name

Md

Last Name

Nojebuzzaman

MiddleName

-

Affiliation

Laboratory of Molecular design and Synthesis, Department of Regenerative Medicine and Applied Medical Science, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan.

Email

-

City

-

Orcid

-

Volume

35

Article Issue

2

Related Issue

7447

Issue Date

2018-12-01

Receive Date

2020-06-17

Publish Date

2018-12-01

Page Start

219

Page End

230

Print ISSN

1110-6581

Online ISSN

2974-4806

Article File

BVMJ_Volume 35_Issue 2_Pages 219-230.pdf

PDF . 1.2MB

Link

https://bvmj.journals.ekb.eg/article_96293.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=96293

Order

22

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

In vivo cloning of plasmids in E. coli is a simpler and faster method for cloning

Details

Type

Article

Created At

22 Jan 2023