The biochemical effect of Chitosan on hepatic lipid metabolism in experimental induced Nonalcoholic fatty
liver disease (NAFLD) in rats was investigated. Thirty male albino rats were divided into three groups (10
rats each). Group I (control group): rats was fed on normal diet. Group II (NAFLD Group): rats fed on
normal diet enriched with 1% cholesterol and 2% coconut oil. Group III (Chitosan treated group): rats fed
on normal diet enriched with 1% cholesterol and 2% coconut oil and supplemented with Chitosan 5%. Blood
samples were collected after 2, 4 and 6 weeks from the onset of chitosan treatment. All serum were directly
used for determination of total cholesterol (TC), triacylglycerol (TAG), low density lipoprotein
cholesterol(LDL-C), very low density lipoprotein cholesterol (VLDL-C), high density lipoprotein
cholesterol (HDL-C), total lipids,(TL) Phospholipids, Non esterified fatty acids (NEFA) , apo-lipoprotein
-B (Apo-B), tumor necrosis factor alpha (TNF-α) , interleukin-6(IL-6 ) . the obtained results showed a
significant increased in serum TC, TAG, LDL-C, VLDL-C,TL, phospholipids, NEFA and Apo-B
concentrations with a marked decrease in HDL-C level .Also, serum TNF-α ,IL-6 was significantly
increase in NAFLD group was observed . The results mentioned that treatment with Chitosan to NAFLD
induced rats showed a significant change and ameliorate in all parameters nearly normal level that indicated
the Hypolypidemic and anti-inflammatory effect of Chitosan in NAFLD induced rats.