The present study was performed on a total of 100 raw buffalo Milk samples from different sources at
El-Menofiya Governorate that the inspected samples were taken in ice box within an a hour for
bacteriological examination. The results revealed that 80 out of 100 samples (80%) were positive for
staphylococcus spp. Out of those 80 samples, 42(53%) were coagulase positive Staphylococci spp while
38(47%) were coagulase Negative Staphylococci spp. Out of those 42 coagulase positive Staphylococci
spp,31 were (73.8%) Staph. aureus. In vitro Gentamycin, Trimethoprim / sulphamethazole, Ampicillin,
Cephadrine were the most proper antibiotic against isolated Staph. aureus. By using PCR Spa virulence
gene was detected in two studied strains while, enterotoxin E was detected only in one strain out of 6
studied strains. Meanwhile, hIyA and enterotoxin (Sea, Seb, Sec, Sed) virulence genes were not detected
in all studied strains