A total of 30 isolate of E.coli isolated from diseased chicken belonged to different serotypes. All isolate
showed resistance to one or more antibiotics by disc diffusion methods. They were investigated against
6 antibiotics from different groups the result were, gentamicin about 46.6%, erythromycin 63.3%,
tetracycline 80%, ciprofloxacin 40%, ampicellin73.3% and florfenicol 53%.3. Detection of resistance
genes by PCR test relieved negative result in all isolated to detected aac(6′ )-Ib-cr gene, while 22 isolate
out of 30 examined E.coli isolate were carried the blaTEM gene on anther hand floR gene was detected
in 29(96%) of E.coli isolate. Also tetA (A) gene was found in 22(73%) of E.coli isolate out of 30
examined isolate. On another hand aadB (gentamicin resistance gene) gene was present in 8(26%).
11(36%) of E.coli isolate were carried the mph (A) erythromycin resistance gene .Correlation between
the presence of resistance gene and the resistance to antibiotics were recorded