A total 100 samples of liver and intestine from broilers (apparently diseased) from 13 poultry farms
were analyzed to detect the incidence of C. perfringes. The samples were examined bacteriologically by
cultivating on CMC and blood agar, typical colonies were identified and biochemically confirmed. The
total number of positive samples was 46 (46%). C. perfringens showed very high sensitivity to
enrofloxacin, high sensitivity to amoxicillin and cefoperazone, but resistant for mupirocin and
azteronam. By making artificial infection for mice, the mice died after 24 hours and by examination
histopathologicaly, it showed liver degeneration and desquamation of epithelial cells of intestine.
Multiplex polymerase chain reaction method (PCR) was amplified for toxin genotyping by using primer
for clostridium perfringes alpha toxin which amplified at 402 bp