This study was aimed to evaluate the effect of dietary supplementation of prebiotic (Bio-Mos®,
mannoligosacchride), probiotic (Bio-Plus^® 2B, Bacillus subtilis and Bacillus licheniformis) and their
mixture on growth, biochemical parameters and immune-hematological responses of rabbits. Sixty four
New Zealand White male rabbits were divided into 2 equal groups. The 1st group was uninfected and
subdivided into 4 subgroups. The 1^st subgroup fed basal diet (Control), the 2^nd, 3^rd and 4^th subgroups fed on
basal diet supplemented with 1 g Bio-Mos, 0.4 g Bio-Plus and 1g Bio-MOS + 0.4 g Bio-Plus / kg,
respectively for eight weeks. The 2^nd group was similar to the 1^st group but experimentally infected with
Pasteurella multocida. The results in 1^st group showed significant increase (P<0.01) in body weight gain,
phagocytic activity (PA), phagocytic index (PI) and total leukocytic counts (TLC) when compared with
control group 1.1. In addition, there was significant decrease in serum total cholesterol, triglycerides and
glucose when compared with control group 1.1. In 2^nd group , the results showed significant increase
(P<0.05) in body weight gain, (P˂0.001) in phagocytic activity and phagocytic index, RBCS count,
PCV, Hb concentration, and number of lymphocytes while TLC and number of heterophils showed
significant decrease(P<0.001) when compared with control group 2.1 .Also there was significant decrease
(P<0.05) in food conversion ratio (FCR), (P<0.01) in total cholesterol and creatinine and (P<0.001) in
number of heterophils, triglycerides, glucose, alanine amino transferase (ALT), aspartate amino
transferase (AST) and urea in all infected groups fed experimental diets compared with control group
2.1.Supplementing the diet with Bio-Mos, Bio-Plus or their mixture decreased the mortality and
improved the adverse clinical signs and post mortem lesions in all experimentally infected groups
compared with infected untreated control group. Our results indicate that rabbits received mixture of pre
and probiotic groups 1.4 and 2.4 recorded the highest value of daily weight gain, PA, PI, TLC and
lymphocytes number and recorded the lowest FCR followed by rabbits received probiotic. Dietary
supplementation of prebiotic and probiotic and their mixture improves cell-mediated immune response,
liver and kidney functions , decreased the mortality and improved the adverse clinical signs and post
mortem lesions in in rabbits experimentally infected with P. multocida.