This study demonstrated the use of MONTANIDE PET GEL A as polymeric adjuvant in locally
prepared inactivated Equine influenza (EI) vaccine. Safety and potency of the prepared vaccine were
studied by inoculation into guinea pigs and horses and evaluated using Haem agglutination inhibition
(HI) test. The vaccine was proved safe and potent for horses and guinea pigs. The mean HI antibody
titers in sera of guinea pigs and horses at 3 weeks post vaccination were (752.4 and 144), respectively.
The antibody titers in vaccinated horses were monitored where they lasted in their sera within the
protective level (80) up to 9 months post vaccination. Therefore, we recommended the use of Montanide
pet gel A as adjuvant for horse vaccines.