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Generating LacZ-reporter transgenic mice to identify α1 (XIX) Collagen (Col19a1) expression in Dermal Papilla Cells

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Last updated: 04 Jan 2025

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Abstract

It is widely accepted the concept that tissue morphogenesis is mediated by reciprocal interactions
between epithelial and mesenchymal cells, whereas the molecular details of these interactions remain
largely elusive. The hair follicle (HF) is a mini-organ whose proper morphogenesis is governed by a
series of interactions between epidermal and dermal cells. Due to its relatively simple structure, the HF
affords an excellent model to decipher the molecular mechanisms of epithelial – mesenchymal
interactions. To explore the molecular mechanisms by which Dermal Papilla (DP) cells regulate hair
follicle formation, we performed a comparative transcriptome analysis and identified numerous genes
preferentially expressed in the DP cells. Among these, a gene encoding type XIX Collagen (Col19a1)
has become the focus of our attention because of its extensive evolutionary conservation. We generated
Col19a1LacZ reporter mice using CRISPR/Cas9 system to induce homologous recombination to
recapitulate Col19a1 expression pattern. In mouse embryos, Col19a1LacZ expression is confined to the
DP cells of hair follicles, ring sinus of whiskers, skeletal muscles and basal keratinocyte layer of skin
epidermis of limbs and tail. Moreover, Col19LacZ started to be expressed at E14.5 in the dermal
condensates of hair germ stage to the DP cells of mature hair follicle but not in the placode stage.
Ongoing generation of Col19a1 null mice will ultimately explore the functional role of Col19a1 during
HF morphogenesis. From this perspective, it is plausible to expect that future studies will provide
additional functional evidence for Col19a1 during development which might be a useful tool to
understand the molecular mechanisms undergoing HF stem cell regulation

DOI

10.21608/bvmj.2016.31408

Keywords

mesenchymal, morphogenesis, Col19a1, Dermal papilla, CRISPR/Cas9, Evolutionary

Authors

First Name

Ahmed

Last Name

Abo-Ahmed

MiddleName

-

Affiliation

Laboratory of Molecular Design and Synthesis, Department of Regenerative Medicine and Applied Medical Sciences, Graduate School of Medicine, Gifu University , Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt

Email

ahmed.ibrahim@fvtm.bu.edu.eg

City

-

Orcid

0000-0002-2300-9839

First Name

Dong-soo

Last Name

Lee

MiddleName

-

Affiliation

Laboratory of Molecular Design and Synthesis, Department of Regenerative Medicine and Applied Medical Sciences, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan.

Email

-

City

-

Orcid

-

First Name

Masatake

Last Name

Osawa

MiddleName

-

Affiliation

Laboratory of Molecular Design and Synthesis, Department of Regenerative Medicine and Applied Medical Sciences, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan

Email

masaosaw@gifu-u.ac.jp

City

-

Orcid

-

First Name

Xujun

Last Name

Han

MiddleName

-

Affiliation

Laboratory of Molecular Design and Synthesis, Department of Regenerative Medicine and Applied Medical Sciences, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan

Email

-

City

-

Orcid

-

First Name

Md

Last Name

Nojebuzzaman

MiddleName

-

Affiliation

Laboratory of Molecular Design and Synthesis, Department of Regenerative Medicine and Applied Medical Sciences, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu 501-1194, Japan

Email

-

City

-

Orcid

-

First Name

Foad

Last Name

Farrag

MiddleName

-

Affiliation

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafr Elsheikh University, Kafr Elsheikh 33516, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Attia

MiddleName

-

Affiliation

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt

Email

mohamed.mitwalli@fvtm.bu.edu.eg

City

-

Orcid

-

First Name

Hatem

Last Name

Bahgat

MiddleName

-

Affiliation

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt

Email

amanyabbass65@gmail.com

City

-

Orcid

0000-0001-7061-8496

First Name

Ahmed

Last Name

Kassab

MiddleName

-

Affiliation

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt

Email

ahmed.kassab@fvtm.bu.edu.eg

City

-

Orcid

-

Volume

30

Article Issue

1

Related Issue

5349

Issue Date

2016-03-01

Receive Date

2019-05-09

Publish Date

2016-03-01

Page Start

380

Page End

395

Print ISSN

1110-6581

Online ISSN

2974-4806

Article File

BVMJ_Volume 30_Issue 1_Pages 380-395.pdf

PDF . 4.9MB

Link

https://bvmj.journals.ekb.eg/article_31408.html

Detail API

https://bvmj.journals.ekb.eg/service?article_code=31408

Order

42

Type

Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Generating LacZ-reporter transgenic mice to identify α1 (XIX) Collagen (Col19a1) expression in Dermal Papilla Cells

Details

Type

Article

Created At

22 Jan 2023