The present study was carried out for detection and identification of the isolated FMDV from infected
tissue samples using serotyping antigen detection ELISA kit (IZSLER ELISA Kit).Thirty six lesion
samples were collected from different localities (Menofia, Gharbia, Kalyoubia, Giza and Sharquia
governorates) during the period from May 2014 to July 2015.The obtained results indicated that
clinically, unvaccinated infected cattle in Gharbia governorate 2014were positive to FMDV infection
serotype O. Samples collected from infected, vaccinated cattle and buffaloes in Kalyoubia governorate
during 2015 were positive to FMDV infection serotype O. Clinically infected cattle and buffaloes in
Menofia governorate during 2015 seven samples were positive to FMDV infection, which serotyped as
five samples were serotype O & two samples were SAT-2. Clinically infected cattle and buffaloes in
Giza governorate during 2015 sixteen samples were positive to FMDV infection, which eight samples
from them serotyped as SAT-2 & seven samples from them were serotype O & one of them was
serotyped A. Clinically infected un vaccinated cattle in Sharquia governorate 2015 three samples were
positive to FMDV infection serotype A. We concluded that large number of both vaccinated and un
vaccinated animals in these governorates affected by FMDV infection by its different serotypes in the
period of the study, and there are different factors affecting prevalence of FMDV as (age, state of
vaccination, type of vaccination, sex, season and species).