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31364

Detection of Virulence factors of Pseudomonas species isolated from fresh water fish by PCR

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Last updated: 04 Jan 2025

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Abstract

This study was conducted on 100 diseased Nile tilapia (O. niloticus) fish of various sizes collected from
different fish markets in Kaliobia Governorate to estimate the prevalence of Pseudomonas infection and
detection of some virulence genes in the isolated P. aeruginosa strains. The results of bacteriological
examination revealed that the prevalence of Pseudomonas septicemia with Pseudomonas species
isolation was 17.0% (17 \ 100 examined fish). These cases were attributed to P. anguilliseptica; P.
aeruginosa and P. fluorescens (14/43.7%; 12/37.5% and 6/18.8%), respectively. In addition, 32
Pseudomonas species were isolated, 11from liver samples (34.4%); 10 from kidney samples (31.2%); 6
from gill samples (18.8%) and 5 from skin samples (15.6%). Moreover, 14 P. anguilliseptica were
isolated with an incidence of 35.7%, 28.6%, 21.4% and 14.3% followed by 12 P. aeruginosa 33.3%,
25%,16.7% and 25% respectively; 6 P. fluorescens 33.3%; 50.0%,16.7% and 0.0% from the liver,
kidney, gill and skin samples respectively. The in-vitro antimicrobial sensitivity test showed that the
isolated Pseudomonas strains were sensitive to gentamycin; enrofloxacin; norfloxacin; ciprofloxacin
and florphenicol. Meanwhile; they were intermediate sensitive for doxycycline; sulfa-trimethoprim;
oxytetracycline; nalidixic acid and streptomycin. In contrast, they were resistant for cefotaxime;
erythromycin; amoxicillin; methicillin; oxacillin and ampicillin. Moreover, the PCR results revealed
that, opr L and exo S virulence genes were detected in all six studied strains (100.0%). Meanwhile, phz
M virulence gene was detected in 5 out of 6 studied strains (83.3%) and tox A virulence gene was
detected in 4 out of 6 studied strains (66.7%) i.e., all studied strains were Ps. aeruginosa and all of them
were virulent strains

DOI

10.21608/bvmj.2016.31364

Keywords

fish, Bacteriological Evaluation, Pseudomonas Species, PCR, oprL, exoS genes

Authors

First Name

Ashraf

Last Name

Abd El Tawab

MiddleName

A.

Affiliation

Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Benha Univ.

Email

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City

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Orcid

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First Name

Ahmed

Last Name

Maarouf

MiddleName

A. A.

Affiliation

Animal Health Research "Benha branch"

Email

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City

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Orcid

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First Name

Nesma

Last Name

Ahmed

MiddleName

M.G.

Affiliation

Animal Health Research "Benha branch"

Email

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City

-

Orcid

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Volume

30

Article Issue

1

Related Issue

5349

Issue Date

2016-03-01

Receive Date

2019-05-08

Publish Date

2016-03-01

Page Start

199

Page End

207

Print ISSN

1110-6581

Link

https://bvmj.journals.ekb.eg/article_31364.html

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https://bvmj.journals.ekb.eg/service?article_code=31364

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22

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Original Article

Type Code

812

Publication Type

Journal

Publication Title

Benha Veterinary Medical Journal

Publication Link

https://bvmj.journals.ekb.eg/

MainTitle

Detection of Virulence factors of Pseudomonas species isolated from fresh water fish by PCR

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Article

Created At

22 Jan 2023