Quercetin (Q) is a common flavonoid, with potent anti‑inflammatory and antioxidant activities. However, its protective role against the hazards of fungicides has been poorly studied. Therefore, the present work aimed at determining the ameliorative potential of Q against testicular damage induced by carbendazim (CBZ) in male rats. In CBZ‑induced testicular damage rats treated with quercetin, it was demonstrated that the activities of the antioxidant enzymes superoxide dismutase (SOD) and glutathione content (GSH) were increased, malondialdehyde (MDA) was inhibited, and interleukin (IL)‑1β, IL-6, Tumor necrosis factor-α (TNF-α) were suppressed. Quercetin additionally enhanced testosterone and cyclic guanine monophosphate (cGMP) levels. These results suggested that the protective role of quercetin prevents CBZ‑induced testicular damage through the free radical scavenging activities, preventing pro-inflammatory cytokine production and improving the male reproductive markers.