Administration of hormones to broiler chickens for performance- enhancing purposes may lead to
deposit of residues in their carcasses. This could be a potential human health hazard up on exposure
to these residues. Therefore this study was performed on sixty chicks (one day old) were reared
under the same condition. At 10 day old age the chicks were divided into 4 groups (control, I/M
administrated group, oral administrated group and withdrawal group). Then hormonal residues were
detected in chick's carcasses and their livers and kidneys using Enzyme-linked immune sorbent
assay (ELISA) method. The results obtained that mean values of estradiol 14.2±0.20, 20.20±0.30
and 22.29±0.70 ppb in muscle, liver and kidneys of chicken, respectively. .The public health
significance and recommendations for the use of hormones were discussed.