This paper aims to investigate the opportunities and challenges of using electric and hybrid vehicles in the tourism transportation sector in Egypt. To achieve this aim, a closed-ended questionnaire was directed to 100 travel agencies in Luxor and Aswan (class A and C) to investigate their opinions concerning positive and negative aspects of actual road tourism transportation means compared to those expected by applying the new hybrid road transportation technology in the Egyptian tourism sector. The study concluded that most travel agencies in Luxor and Aswan are aware and willing to positively participate in implementing the Egyptian vision of sustainable green road transportation by using electric and hybrid vehicles instead of gasoline-based vehicles. Having legislative and economic incentives could influence them to participate more positively.