Many hotels are subject to environmental regulations. A growing practice of the impact of activities on the environment has created a greater need to take into account environmental factors housekeeping department, because this department is most important department for application environmental practice, therefore, an increasing number of first class hotels in Alexandria are certifying their environmental management systems (EMS) by the ISO 14001 series standards. Improving the environmental practice of hotels is one way of limiting the environmental damage. EMS provides a framework for organizations that wish to effectively manage their environmental affairs. Implementing an EMS that conforms to the ISO 14001 standard, therefore, this research aims to assess the environmental practices in housekeeping department, because it is considered one of the most important departments related to environmental management inside in hotel and implementation the environmental practice also the largest department containing the largest employees.To achieve this objective, the researcher surveyed 300 employees in upscale hotels in Alexandria. Data were analyzed using Statistical Package for Social Science (SPSS 22). To high light of these results, the researcher recommended the need to pay attention to the application of environmental practices and increase the awareness of employees of good practices of (Energy Saving, Water Conservation and Waste Management).