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267523

Isolation and Identification of Helicobacter spp. From Gastric Biopsies and Autopsies in Human and Animal Gastric Patients

Article

Last updated: 04 Jan 2025

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Microbiology & Immunology

Abstract

Helicobacter infection is a major public health issue worldwide, about 4.4 billion individuals worldwide were confirmed to be infected. Helicobacter is a human pathogen that is transmitted from human to human and has a zoonotic relationship. Helicobacter gastritis, peptic ulcer, and perforation may lead to cancer. This study aims to try to isolate the Helicobacters from gastric biopsies and autopsies in human patients and animals (dogs and cats).
Methodology:105 gastric biopsies were collected from dyspeptic patients and 5 gastric autopsies were collected from dogs and cats. The samples were cultivated and characterized by microbiological and molecular tools (PCR).
Results: The results showed few harvested isolates (n=22) after characterization the confirmed Helicobacter isolates were 10 only from humans and animals.
Conclusion: The conclusion is the microbiological method is so costive, time-consuming, and less sensitive. The presence of accurate and rapid tests is so crucial for specific and sensitive detection of Helicobacters.

DOI

10.21608/jcvr.2022.267523

Keywords

Gastric biopsies, Helicobacter, isolation, PCR

Authors

First Name

Hamed

Last Name

Wafy

MiddleName

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Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, University of Sadat City, Egypt.

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First Name

Shymaa

Last Name

Abdelmalek

MiddleName

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Affiliation

Microbiology department, Faculty of Veterinary Medicine, Cairo University.

Email

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City

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Orcid

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First Name

Alaa Eldin

Last Name

Mostafa

MiddleName

H.

Affiliation

Department of Microbiology, Faculty of Veterinary Medicine, University of Sadat City, Egypt.

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Orcid

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Volume

4

Article Issue

2

Related Issue

37415

Issue Date

2022-10-01

Receive Date

2022-10-29

Publish Date

2022-10-01

Page Start

159

Page End

165

Print ISSN

2636-4018

Online ISSN

2636-4026

Link

https://jcvr.journals.ekb.eg/article_267523.html

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https://jcvr.journals.ekb.eg/service?article_code=267523

Order

267,523

Type

Original Article

Type Code

799

Publication Type

Journal

Publication Title

Journal of Current Veterinary Research

Publication Link

https://jcvr.journals.ekb.eg/

MainTitle

Isolation and Identification of Helicobacter spp. From Gastric Biopsies and Autopsies in Human and Animal Gastric Patients

Details

Type

Article

Created At

22 Jan 2023