Recently, there are increasing number of reports that suggests the microvesicles (MVs) derived from mesenchymal stem cells (MSCs) as a beneficial therapeutic treatment as a cell free regenerative option for multiple diseases so the current study aimed to evaluate the efficacy of MSCs-derived MVsin potentiation of the healing process of surgically repaired,induced Achilles tendon rupture in dog.Study design:Twelve adult mongrel dogs of both sexes weighing 15–18 kg and aged 2-3 years were used in this study and were divided into two groups, Control group: with no additional treatments. MVs group: MSCs-derived MVs were used as additional treatment. During observation period,dogs were observed clinically at 3,6, 8, 10- and 12-weeks post-operation and histologically at 6- and 12-weeks post-operation.Results: the clinical observation revealed a great significant difference between the two groups and the MVs could make complete healing at the end of the observation period with full weight bearing capacity and no lameness during walk and histological examination revealed significantly faster healing rate, better arrangement of the fibers and higher collagen expression.Conclusion: The present study revealed that MVs have a great ability to enhance and accelerate the healing and regeneration processes of the Achilles tendon