COD is an ovarian dysfunction and a major cause of reproductive infertility in dairy cattle. The objective of this study was to compare therapeutic approaches of COD in Holstein dairy cows and its effect on pregnancy/ artificial insemination (PR/AI). Those cows classified into 8 groups where 1stgroup (n=10)  received (GnRH+hCG+PRID)  , 2ndgroup (n=10) received  (GnRH+hCG+PRID 2) , 3rdgroup (n=7) received (GnRH + hCG + CIDR), 4thgroup (n=46), received (GnRH+CIDR),5thgroup (n=10) received (GnRH+hCG), 6^th group (n=19) received (GnRH) , 7^th group (n=30), received (GnRH + PRID) and 8^th group (n=10), received (GnRH + hCG + PRID1). Result revealed that differences in PR/AI among different treatment protocols were not significantly different (Chi square = 5.29, P = 0.63), where PR/AI in 8 groups was (50%, 50%, 57.14%, 50%, 30%, 52.63%, 66.67% and 40% respectively). In conclusion, the highest pregnancy rates numerically were achieved in cystic ovarian disease cows treated using single GnRH injection and PRID (66.67%), while the lowest pregnancy rates were reported in cows treated using GnRH and hCG (30.00%).