The objective of this study was to compare therapeutic approaches of repeat breeder cases in Holstein dairy cows and its effect on pregnancy rate/artificial insemination (PR/AI). The repeat breeder cows (n=62) which received intrauterine infusion of penicillin-streptomycin combination (Pentomycin) after A.I which yielded the highest pregnancy results (68.33%), while intrauterine benzyl penicillin to repeat breeder cows (n=32) lead to establishment of pregnancy at a rate of (31.25%) of treated cows. Insertion of CIDR on 5^th day after A.I in repeat breeder cows (n=25) was accompanied by (52%) PR/AI, and, a twice used PRID2 in cows (n=12) gave satisfactory PR/AI (41.67%). Single administration of hCG 2500 IU (n=47) on 5^th day after A.I was accompanied by an increased PR/AI to (89.36%). In spite of that a single dose of 3300 IU hCG (n=126) on day 5 after AI decreased PR/AI in treated cows to (48.41%) and the same trend was observed in repeat breeder cows which received 5000 IU hCG n=(17) single dose (47.05%). Moreover, repeated administration of a small dose of hCG 1000 IU on days 4, 5 and 6 after AI (n=104) gave (60.58%) PR/AI.