Effects of long transportation (~ 900 km - Egypt) stress with or without the use of antistress formula on H/L ratio, stress hormones and plasma antioxidant enzymes were carried out in Japanese quail. The transported quail separated into two groups: G2 was the non-treated transported group, G3 was the treated group (given the antistress formula 48 h before and 24 h after transportation). The samples of blood collected before (G1) and after (G2 & G3) transportation. The G2 significantly had a higher heterophils %, H/L ratio, T4, malondialdehyde level, and corticosterone hormone concentration immediately on arrival and 24 h after transportation compared to G3. On the other hand, the results of the G3 significantly had a higher lymphocyte % and glutathione level immediately on arrival and after 24 h after transportation compared to the untreated group (G2). The total quail death throughout 24 h after 900 Km transportation were 21% (210 quail) for the G2 and 4% (40 quail) for the G3. The results indicate that the SH- antistress formula can help quail to better resist long-term transportation disorders which were reflected on the quail's physiological stability and avoid economic losses of poultry industry.