The kinetic disposition of lincomycin in broiler chickens was studied after single oral administrations at a dose of 10 mg /kg b.wt. Serum concentrations of lincomycin were determined by using reverse phase HPLC. Following compartmental analysis, a two-compartment open model best described the concentration-time data of lincomycin after oral administration. After oral administration, the drug reached its maximum serum concentrations (C_max) of 5.892 ± 0.060 μg/ml at maximum time (T_max) of 1.241 ± 0.015 h, absorption half-life (t_1/2ab) and (AUC_0-t) were 0.347 ± 0.016 h and 48.868 ± 0.175 μg/ml.h respecrtively. Lincomycin was eliminated at a rate (K₁₀) equal to 0.147 ± 0.006 h and cleared by all clearance processes (Cl/F) in the body at rate of 0.312 ± 0.004 (mg/kg)/(μg/ml)/h. In conclusion, lincomycin administered orally at dose of 10 mg/kg b.wt showed a good pharmacokinetic profile with a long elimination half-life (t_1/2β) 7.041 ± 0.402 h.