Objective of this study to assess the efficacy of platelet rich plasma (PRP) in the treatment of extensive corneal ulcers in albino rabbits with two different administration techniques. A total of 24 rabbits were used in this study. After collecting intracardiac blood samples, platelet-rich plasma was obtained by double centrifugation. Alkali burns were inflicted on the central corneas of all rabbits using a sterile swab soaked in 1 M NaOH for 60s. Only Left eye in each rabbit was used. Animals were divided into 4 groups. GroupI (n=6) normal control group received PRP drop twice daily with 8-h intervals for 7 successive days. whereas group II (n = 6) did not receive any treatment and served as the control untreated group. The third group (n = 6) received only one-shot of subconjunctival PRP. The fourth group (n=6) received PRP drop twice daily with 8-h intervals for 7 successive days. Corneal histopathology was investigated for epithelial regeneration, presence of inflammation, and structural integrity of fibroblasts. Data were expressed as mean ± standard deviations. Data from experimental groups were statically analyzed using one-way ANOVA with Turkey's post hoc multiple comparisons tests using the GraphPad Prism software version 5 (San Diego, CA, USA) and P