Corneal ulcer is considered one of the most common ocular surface diseases, it may lead to blepharospasm, photophobia, lacrimation, conjunctival hyperemia, and corneal oedema. Conventional treatments are sometimes not effective in solving the pathological condition therefore blood derivatives which are rich in growth factors were used to accelerate wound healing as well as its ability to induce regeneration of affected tissues. Blood derivatives include autologous serum (AS), eye drops platelets rich plasma (E-PRP). A 6 mm induced corneal wound were performed at the center of the cornea at one eye at a 240 M depth in 20 mongrel dogs. Dogs divided into three groups, E-PRP group (8 dogs) and AS group (8 dogs) and a control group (4 dogs) received normal saline eye drops. All dogs were received 4-time treatment per day until negative fluorescein stain test is obtained. Histological study was performed for 3 dogs (one from each group) two months from the beginning of the experiment. Clinically the E-PRP group showed faster healing time with a significant difference (3.62±0.23 day) from AS group (4.75±0.25 day) and control group (6.75±0.30 day). Histologically, E-PRP group showed more arrangement of the epithelial layers compared with AS group and control group. Aim of our study: to compare the efficacy of AS and E-PRP in the healing of induced corneal wound in dogs. In conclusion, E-PRP and AS showed a significant faster epithelial healing from control group. As well as E-PRP showed also a significant improvement over AS in heling time and quality of regenerated epithelial tissue.