The pharmacokinetic profile of thiamphenicol was studied in broiler chickens following a single intravenous (IV) and oral (PO) administration at dosage rate 30 mg/kg BW. Serum concentrations of TP were determined by high performance liquid chromatography (HPLC). After IV dose, the serum thiamphenicol concentration time course was found to obey two-compartment open model. After IV dose, elimination half-life (t1/2λz), volume of distribution at steady state (Vdss), total body clearance (Cltot) and mean residence time (MRT) of TP were 4.58±0.2hr, 2.31±0.1L/kg, 0.31±0.006L/hr/kg, and 2.44±0.1hr, respectively. After oral administration of thiamphenicol, the peak plasma concentration (Cmax) was 14.58±0.1μg/ml and was obtained at 3.64±0.01hr (tmax) post administration. Elimination half-life (t1/2el) and absorption half-life t1/2ab.) were 2.65±0.01hr and 2.06±0.01hr, respectively. The systemic bioavailability following oral administration of TP was 117.79±1.2%. TP therapy with dosage rate of 30 mg/kg BW is suggested for a beneficial clinical effect in broiler chickens.