The efficacy of Bacillus subtilis & Bacillus licheniformis Mixture (BAM-100®, recommended dose; 100 gram / ton feed) as probiotic dietary supplementation on the performance parameters (body weight gain, food consumption, food conversion as well as mortality), the hematological profile and serum biochemical parameters in broiler chickens was investigated. A total of 40 one‐day‐old broiler chicks (body weight: 43.35±0.25 g) were randomly divided into two treatments, the two treatment groups were non-supplemented control group and BAM-100® supplemented group. Dietary BAM-100® supplementation significantly improved weight gain (WG), feed conversion ratio (FCR) and mortality compared to non-supplemented control treatment. WBCs, RBCs, PCV, Hb, MCV, MCH and LYM were showed a significant increase in supplemented group that fed on probiotic compared to non-supplemented control group.