Healing of surgical or traumatic wounds in equine usually delayed and complicated. Moreover, the incidence of traumatic wounds in equine are greater than any other animals, therefore, a new therapy are required for a fast and satisfactory healing. In this study we aimed to achieve optimal healing with minimal or even no scar formation. Platelets Rich Plasma (PRP) which play an important role in wound healing due to its massive content of growth factors. In this work, we used 6 donkeys divided into 2 equal groups. In all animals, one skin wounds were created bilaterally on the back region (3×3 c, full-skin thickness). The first group received one PRP injection in the right wound directly after wounding, and saline treatment to the left wound daily. The second group received tow PRP injection in the right wound, the first directly after wounding and the second at the 14th day after wounding, while the wound of left side subjected to betadine treatment daily. Clinically, there was no great difference between single or double shout of PRP injection. The contraction occurs at 14th day, but increased after the second shout of PRP injection, while it was delayed in both saline and betadine treated groups. PRP treated groups showed complete wound closure with healthy granulation tissue, unlike the other groups. Microscopically: PRP treated wounds revealed complete epidermal and dermal formation with skin appendages. Only the periphery of wounds covered with epidermis while the center still denuded in both saline and betadine treated groups.