The present study was undertaken to investigate and phenotypically characterize serotypes and antibiotic susceptibility of Salmonella isolates prevalent in broiler chicks. In this study a total of 26 broiler farms allocated in El-Gharbia and El-Menofia Governorates during the period of November 2015 to November 2016. The percentage of Salmonella positive samples were 15.4%, the following serotypes could be isolated and identified Salmonella Pullorum (n=1), Salmonella Havana (n=1), Salmonella Durham (n=1), and Salmonella Entritidis (n=1) from broiler chicks. In the present study, most of the tested Salmonella serovars were multi-drug resistant (MAR) and had high MAR indexes against the commonly used antibiotics in the poultry industry in Egypt.