The present work aimed to study the protective effect of a locally prepared combined freeze-dried vaccine of M. haemolytica, P. trehalosi and polyvalent Clostridial vaccine. Evaluation of such preparation following the quality control tests revealed that, it was stable, free from foreign contaminants, safe and immunogenic .The prepared vaccine was evaluated in rabbits and the immune response of vaccinated animals to combined vaccine was estimated by ELISA test for M. haemolytica, P. trehalosi antibodies. High antibody levels induced for M. haemolytica and P. trehalosi in combined freeze-dried vaccine compared with combined liquid one. There was a good antibody response to all Clostridial components determined by Serum neutralization test (SNT) and agglutination test in sera of rabbits revealed no substantial differences with respect to the antibody values between combined freeze-dried vaccine and combined liquid vaccine. The obtained results confirmed that the locally prepared combined freeze-dried vaccine ofM. haemolytica, P. trehalosi and polyvalent Clostridial vaccine was of good quality and producing high antibody titers.