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36570

Molecular Detection of Some Virulence Genes of Salmonella Serotypes Isolated From Poultry in Egypt

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Last updated: 04 Jan 2025

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Abstract

Salmonellosis is recognized as an important health problem and a major challenge in poultry industry worldwide. This study highlight the prevalence rate of salmonella serotypes in  chickens as well as demonstration of some critical virulence genes by using specific primers sets in PCR reaction. Out of 100 freshly dead chickens, sixteen chicken samples were positive to Salmonella with 16% prevalence rate.The rate of recovery of Salmonellae from the different internal organs showed that the intestine is the most predication seat followed by, heart and liver with (8%), (6%) and (2%), respectively. Salmonella isolates (16) from chickens were serotyping using O and H antigen. The results revealed that S. Enteritidis was the most serotype found in 5 isolates (31.25%) followed by S.Infantis in 4 isolates (25%). Both S.Newlands and S.Kentucky found in 2 isolates 12.5%. Each of S.Ferruch, S.Weybridge and S.Naestved have one isolate (6.25%).The  application of PCR for detection of invA, avrA and stn virulence genes in nine salmonella serovars revealed that these genes (invA, avrA and stn)were detected in 100% of tested strains except S. Naestved does not contain stn gene. Hence, this study recommends that PCR may be used for rapid and sensitive detection of Salmonella and help in understanding the role of these genes in pathogenicity of salmonella infection in poultry.

DOI

10.21608/jcvr.2019.36570

Keywords

Salmonella, PCR, invA, stn, avrA gene, Serotypes

Authors

First Name

Marwa

Last Name

ElSheikh

MiddleName

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Affiliation

Veterinarian at the Directorate of Veterinary Medicine, Menofiuya province

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City

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Orcid

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First Name

Eman

Last Name

Abdeen

MiddleName

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Affiliation

Bacteriology, Mycology and Immunology department, Faculty of Veterinary Medicine, University of Sadat City

Email

eman.abdeen@vet.usc.edu.eg

City

-

Orcid

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First Name

Ahmed

Last Name

Ammar

MiddleName

-

Affiliation

Microbiology department, faculty of Veterinary Medicine,Zagazig University

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Volume

1

Article Issue

1

Related Issue

5738

Issue Date

2019-06-01

Receive Date

2018-12-19

Publish Date

2019-06-01

Page Start

86

Page End

93

Print ISSN

2636-4018

Online ISSN

2636-4026

Article File

JCVR_Volume 1_Issue 1_Pages 86-93.pdf

PDF . 1.5MB

Link

https://jcvr.journals.ekb.eg/article_36570.html

Detail API

https://jcvr.journals.ekb.eg/service?article_code=36570

Order

16

Type

Original Article

Type Code

799

Publication Type

Journal

Publication Title

Journal of Current Veterinary Research

Publication Link

https://jcvr.journals.ekb.eg/

MainTitle

Molecular Detection of Some Virulence Genes of Salmonella Serotypes Isolated From Poultry in Egypt

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Article

Created At

22 Jan 2023