A total of 60 one day old broiler chicks (Cobb 500) were used, the chicks were first weighted and randomly assigned into three experimental groups (20 chicks per each), the first group (G1) was negative control and received basal diet, the second one (G2) was a positive control and received basal diet and challenged orally with mixed culture of Salmonella Pullorum and Salmonella Gallinarum (1x10⁸/ml) at 25 days of age, the third one(G3) received the probiotic mix from 1 day of age this mix contained: Bacillus subtilis MORI 91 [2×10⁸colony forming unit (cfu)/g], Clostridium butyricum M7 (2.06×10⁸cfu/g), Lactobacillus plantarumK34 (2×10⁸cfu/g),and challenged orally with mixed culture of S.Pullorum and S.Gallinarumat 25 days of age. The results of the cytokines and inflammatory markers were detected as follows: the first group gave (105, 89.5, and 68.1), the second group gave (160,135, and 115), and the third group gave (85, 65.3, and 56.3) for IFN- γ, IL12, and IL4 respectively. The results of body weights and feed conversion ratios were: the first group gave (1550 and1.91), the second group gave (1300 and 1.8), and the third group gave (1800 and 2.01) respectively. Furthermore, the results of mortalities, and PM lesions were: the first group showed 4/20 (20%) dead chicks with enteritis, while  the second group showed7/20 (35%) dead chicks with enteritis and enlargement with necrosis of the liver, spleen and kidneys, and the third group  showed 2/20 (10%) dead chicks with enlargement of the liver, spleen and kidney. For the hematological parameters the first group gave (30.65 ± 0.85, 2.82 ± 0.31, and 9.25 ± 0.16), the second group gave (28.26± 0.76, 2.62 ± 0.23, and 8.75± 0.26), and the third group expressed (34.85± 1, 3.66 ± 0.25, and 10.5.13 ± 0.37) for PCV, mean RBCs (10⁶ µl^-1), and hemoglobin, respectively. There was a significant difference in the results of cytokines, body weight, feed conversion ratios, mortality rate, PM lesions, and hematological parameters between the probiotic treated and the control groups with P < 0.05. It was concluded that the probiotic mix enhanced the growth and hematological parameters, also it lowered the inflammatory markers and the mortalities.