Onychomycosis is a common nail disease, especially in older patients. Various treatment options are currently available for onychomycosis; however, their limitations include high failure rates, time-consuming nature, high cost, and high risk of drug interactions. Objective: To evaluate the efficacy of onychomycosis treatment with a long-pulsed 1064-nm Nd:YAG laser. Patients and Methods: ten patients were assessed. The study involved treatment with a long-pulsed 1064-nm Nd:YAG laser in two sessions at 4-week intervals. Fungal culture at two media sabourauds dextrose agar with cycloheximide and without cycloheximide and microscopic examination were performed at the start and then a one-month after the second session. Results: Of all ten patients after two sessions, the mycological test results were negative in eight (80%). The result showed that the mean improvement percentage was 60.50 %. Side effects were mild and limited to mild pain and slowing of the growth of nails after the laser procedure. Conclusions: Long-pulsed 1064-nm Nd:YAG laser therapy is safe and effective for treating onychomycosis.