Background: Aging-related changes in liver alter both hepatic structure and function thus, increasing the mortality rate in susceptible old patients. Metformin provide health benefits to elderly individuals when ingested in appropriate amounts and it is one of the physiological triggers of Peroxisome Proliferator- Activated Receptor- Gamma (PPARγ) and irisin Aim: This study aimed to investigate the effect of metformin on hepatic aging induced by D- galactose (D- gal) in rats clarifying the role of PPARγ/ irisin pathway in this process. Methods: 24 adult Wister albino male rats divided into 4 groups: group I (control group): rats received saline; ip, group II (D- gal group): rats received D- gal; ip, group III (Metformin group): rats received Metformin orally & group IV(Metformin + D- gal group): rats received D- gal with Metformin.
At the end of expriment, The serum samples were taken for biochemical estimation of Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Albumin and hepatic PPARγ and irisin protein levels Results: compared to control group, D- gal caused hepatic injury confirmed by a significant increase in ALT and AST with a significant decrease in Albumin. Metformin in group IV prevented these changes through increased hepatic PPARγ and irisin. Conclusion: Metformin showed a protective effect against the D- gal induced hepatic aging through induction of hepatic PPARγ and irisin.