Background and study aim. Non alcoholic fatty liver disease has an increasing prevelance worldwide.It is closely assosciated with metabolic syndrome. The aim of this study is to assess the assosciation between non alcoholic fatty liver disease and serum level of Vitamin D. Patients and Methods: This cross sectional study was conducted on 95 subjects devided into 2 groups (1st group 45 patients with sonographically proven Non alcoholic fatty liver disease and 2nd group 50 healthy subjects). All the patients were evaluated by thorough full history taking, clinical examination, routine laboratory investigations, lipid profile,abdominal ultrasound,serum Vitamin D by ELISA.Results.Mean weight, Body mass index and waist circumference were significantly higher in patients with severe steatosis (P value 0.001,0.000,0.002 respectively).Incidence of Diabetes mellitus, hypertension and cardiovascular diseases were significantly higher in patients with non alcoholic fatty liver disease than those without (P value 0.001,0.003 and 0.009 respectively)and they significantly increased with increasing grades of fatty liver.Serum level of Vitamin D was significantly lower in Non Alcoholic Fatty Liver Disease patients than those without ( P value