Background: Neutrophil Gelatinase- Associated Lipocalin (NGAL) is a useful clinical biomarker for early diagnosis, predicting disease severity, therapeutic monitoring, and for predicting clinical outcomes. Aim: The current study aimed to investigate  serum   Neutrophil gelatinase-associated lipocalin ( NGAL ) levels as a marker of malnutrition in maintenance hemodialysis patients. Subjects and methods: This study was conducted on 50 subjects. Subjects were divided into three groups .Group (1): Hemodialyzed patients : Included 30 patients before starting hemodialysis (HD). Group (2):  The same 30 patients after hemodialysis. Group (3): Included control group. Serum levels of NGAL was measured by  enzyme-linked immunosorbent assay (ELISA ). Results: There is high statistically significant decrease in NGAL in dialyzed patients after dialysis when compared to the same patients before dialysis (p < 0.001). There is  statistically significant positive correlation between NGAL after dialysis and albumin, pre-albumin, serum iron, serum ferritin, TLC and neutrophils %. There is a statistically significant decrease in the serum level of NGAL (before and after) in dialysis patients with malnutrition  when compared to ( before and after )  dialysis patients without  malnutrition. Conclusion: Serum level of NGAL seems to be a reliable marker of malnutrition appearance, allowing early diagnosis and preventive therapy.