Background: PROMs occurs in 10% of all term pregnancies and about 2- 4% of preterm pregnancies and is associated with complications such as infection and preterm birth. Vaginal fluid urea and creatinine levels may be helpful in the diagnosis of PROM. Objectives: To evaluate the reliability of vaginal washing fluid urea and creatinine as biomarkers for diagnosis of preterm PROM. Subjects and Methods:A prospective case control study was conducted at the Obstetrics and Gynecology Department; Benha University Hospital during the period from September 2018 to September 2019. The study included 100 cases: 50 inpatient cases with preterm PROM and 50 cases from those attending outpatient clinics for routine antenatal care. All patients underwent speculum examination, nitrazine paper test, and U/S. Results: The creatinine levels were 0.35 ±0.12 mg/dl and 0.16± 0.08 mg/dl in confirmed cases and controls, respectively. The urea levels were 6.5 ±2.48 mg/dl and 3.4 ±1.68 mg/dl in confirmed cases and controls, respectively. There was a highly significant difference regarding creatinine level and urea level (P < 0.001). Sensitivity, specificity, PPV and NPV for creatinine level were 100%, 84%, 86,2% and 100%, respectively. Sensitivity, specificity, PPV and NPV, for urea level was 100%, 80%, 83.3% and 100%, respectively, with a cut‑off value of 0.2 mg/dl for creatinine level and 3 mg/dl for urea level. Conclusions: Detection of vaginal fluid urea and creatinine to diagnose PROM is a simple, reliable and rapid test with high sensitivity, specificity, PPV, NPV.