Background:  It is considerable that chronic kidney disease–mineral bone disorder (CKD-MBD) is a vital issue in chronic kidney disease patients. Parathyroid hormone (PTH) is a fundamental factor in the pathogenesis of this disorder in dialysis patients. It is believed that plasma magnesium (Mg²⁺) has an essential role in the regulation of PTH secretion in a manner which is slightly similar to calcium. Objectives: Illustrates the relationship between serum magnesium and serum parathyroid hormone in hemodialysis patients. Patients and Methods: A cross-sectional study had been carried out in the Hemodialysis Unit, Zagazig University Hospitals, Faculty of medicine, Zagazig University from the period between December 2018 and May 2019. The study included (77) participants diagnosed with end stage renal disease on regular hemodialysis. Results: There is no statistically significant correlation between serum magnesium and serum parathyroid hormone. Conclusion: Our findings suggest that factors other than serum Mg may be more important in the regulation of PTH secretion and action. Our findings require further investigations with larger and multicentric studies.