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265107

COMPARATIVE RESULTS OF RAPD-PCR GENETIC POLYMORPHISM OF SCHISTOSOMA HAEMATOBIUM

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Last updated: 04 Jan 2025

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Abstract

Schistosomiasis is one of the public health threats in Egypt. S. hematobium infection significantly increases the risk of urinary bladder metaplasia and cancer. The study of the genetic diversity of S. hematobioum might help predict the severity of the disease and the resistance to treatment. In the present study, the DNA of the S. hematobium was amplified using RAPD-PCR from 27 urine samples and in addition, a positive control sample was used. We used RAPD-PCR primers (A01, A02, A12, A13, and Y20). DNA electrophoresis was done on a 1.2% agarose gel and samples were run with high molecular weight and a low-molecular weight100pb ladder. The samples generated a total of 182 bands with 5 primers. The average band numbers were 3 with primer A01, 2 with primer A02, 1 with primer A12, 3 with primer 3, and 2 with primer Y20. The average molecular weight for the generated bands with each primer was 938pb, 738pb, 1425pb, 1006pb, and 866pb respectively. Our results show genetic polymorphism in the samples specifically in primers A02, A13, and Y20. This genetic polymorphism was evident by the differences in the band numbers and weights (allelic composition). Further research is required to assess the relationship between genetic polymorphism and pathology caused by S. hematobium infection and finally the response to treatment.

DOI

10.21608/smj.2022.156011.1341

Keywords

S. hematobioum, RAPD-PCR, genetic polymorphism

Authors

First Name

Shaimaa Mukhtar

Last Name

Abdelwahed

MiddleName

-

Affiliation

Medical parasitology department, Sohag Faculty of Medicine, Sohag University

Email

shaimaa.mukhtar@gmail.com

City

-

Orcid

-

First Name

NADA

Last Name

NADI

MiddleName

-

Affiliation

Medical Parasitology Department, Sohag Faculty of Medicine

Email

nadanadi295@gmail.com

City

-

Orcid

-

First Name

EMAN

Last Name

OMRAN

MiddleName

-

Affiliation

Department of Medical Parasitology, Sohag Faculty of Medicine, Sohag University. Egypt

Email

moabdallah.1998@hotmail.com

City

-

Orcid

-

First Name

AMAL

Last Name

ABD ELMAWGOOD

MiddleName

-

Affiliation

Department of Medical Parasitology, Sohag Faculty of Medicine, Sohag University.

Email

am.ahmed_1977@yahoo.com

City

-

Orcid

-

Volume

26

Article Issue

3

Related Issue

37168

Issue Date

2022-09-01

Receive Date

2022-08-21

Publish Date

2022-09-01

Page Start

20

Page End

27

Print ISSN

1687-8353

Online ISSN

2682-4159

Link

https://smj.journals.ekb.eg/article_265107.html

Detail API

https://smj.journals.ekb.eg/service?article_code=265107

Order

265,107

Type

Original Article

Type Code

785

Publication Type

Journal

Publication Title

Sohag Medical Journal

Publication Link

https://smj.journals.ekb.eg/

MainTitle

COMPARATIVE RESULTS OF RAPD-PCR GENETIC POLYMORPHISM OF SCHISTOSOMA HAEMATOBIUM

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Article

Created At

22 Jan 2023