41679

Prevalence of gyrA and parE mutations in clinical isolates of Streptococcus pneumoniae with decreased susceptibilities to different Fluoroquinolones

Article

Last updated: 04 Jan 2025

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Abstract

Introduction: Streptococcus pneumoniae is a major Gram-positive pathogen responsible for pneumonia, bactermia, otitis media, and meningitis leading to considerable morbidity and mortality among children and elderly individuals. The primary goals of antibiotic treatment of respiratory tract infections are clinical efficacy of treatment, pathogen eradication, and prevention of resistance development. Resistance to fluoroquinolones in S. pneumoniae arises in a stepwise fashion and results from alterations in the target binding site due to the acquisition of spontaneous mutations in the quinolone resistance-determining regions (QRDRs) of the topoisomerase IV and DNA gyrase genes. Although mutations usually occur in the QRDRs of parC and gyrA, a role for mutations in the parE subunit in low-level resistance has been reported.
Aim of the work: The aim of this study was to determine the prevalence of fluoroquinolone resistance Streptococcus pneumoniae (FQRSP) and to examine the genetic relatedness of pneumococcal isolates with parE and gyrA genes mutations in different specimens in Sohag University Hospital.
Patients and Methods: This study was prospectively conducted over a period of 24 months between October 2015 and September 2017, at Sohag university hospital. During the study period, 78 patients hospitalized for a syndrome consistent with a diagnosis of community acquired pneumonia (CAP ) included in this study with a mean age of 34.5 years (range, 2 to 67), 60% of whom were males. A CAP syndrome was defined as a newly recognized pulmonary infiltrate together with 2 of the following findings: subjective fever or documented temperature 37.4 °C, increased cough, sputum production, or shortness of breath, pleuritic chest pain, confusion, rales, leukocytosis, (according to age) (1). Patients who had taken antibiotic treatment within 3 days prior to initial visit were excluded from this study.
Results: Our study illustrate the role of mutation in the gyrA&parE genes and the effect of mutations in the both genes in fluoroquinolone resistance among S. pneumoniae isolates.
Conclusion: The present study provide an opportunity to view the predominant mutations conferring reduced susceptibility to FQs in clinical pneumococcal isolates. There is a strong relationship between these mutations and decrese susceptibility to the most fameous FQs to some extent, although this varies between strains and for each drug.

DOI

10.21608/smj.2018.41679

Keywords

gyrA, ParE, Streptococcus pneumonia, Fluoroquinolones

Authors

First Name

Laila

Last Name

Yousef

MiddleName

M

Affiliation

Department of Clinical and Chemical pathology, Faculty of Medicine, Sohag University.

Email

lailamohamed@med.sohag.edu.eg

City

Sohag

Orcid

-

First Name

Ghada

Last Name

Ismael

MiddleName

A

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Ain Shams University.

Email

-

City

Cairo

Orcid

-

First Name

Ashraf

Last Name

Mohammed

MiddleName

k

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Sohag University.

Email

ashraf_abdallah@med.sohag.edu.eg

City

Sohag

Orcid

-

First Name

Mohamed

Last Name

Mahmoud

MiddleName

H

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Sohag University.

Email

-

City

Sohag

Orcid

-

Volume

22

Article Issue

1

Related Issue

4799

Issue Date

2018-01-01

Receive Date

2018-12-03

Publish Date

2018-01-01

Page Start

229

Page End

241

Print ISSN

1687-8353

Online ISSN

2682-4159

Link

https://smj.journals.ekb.eg/article_41679.html

Detail API

https://smj.journals.ekb.eg/service?article_code=41679

Order

38

Publication Type

Journal

Publication Title

Sohag Medical Journal

Publication Link

https://smj.journals.ekb.eg/

MainTitle

Prevalence of gyrA and parE mutations in clinical isolates of Streptococcus pneumoniae with decreased susceptibilities to different Fluoroquinolones

Details

Type

Article

Created At

22 Jan 2023