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44876

Immunohistochemical, cellular localization and expression of inhibin hormone in the buffalo (Bubalus bubalis) adenohypophysis at different ages

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Last updated: 04 Jan 2025

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Abstract

  The pituitary adenohypophysis was obtained from thirty buffaloes-cows, their age's ranges from one month to12 years. Sections of adenohypophysis tissues were immunostained for α, βa, and βb subunits of inhibin hormone. Positive immuno-staining specific for the α subunits of inhibin were detected in the cells of the follicles of the adenohypophysis in all ages. Moreover, immunostaining specific for the inhibin βa subu- nits were strong positive at one month, weak positive at 4.5, 8 and 12 years and were negative at 8 months and 1.5 years. However, the immunostaining specific for the in- hibin βb subunits were positive at one and 8 months, 8 and 12 years and weak positive at 4.5 years and negative at 1.5 year. RT-PCR analysis revealed that both α and βb subunits are expressed in all ages except at 1.5 years old animals while βa subunit is only expressed at young age.  

DOI

10.21608/jva.2012.44876

Keywords

Inhibin, Buffalo-cows, Adenohypophysis, RT-PCR, Immunohistochemistry

Authors

First Name

H.

Last Name

Attia

MiddleName

F.

Affiliation

Department of Histology and Cytology, Faculty of Veterinary Medicine, Benha University, Egypt.

Email

hossam222@yahoo.com

City

-

Orcid

-

First Name

M.

Last Name

Kandiel

MiddleName

M.

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Egypt.

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City

-

Orcid

-

First Name

T.

Last Name

Ismail

MiddleName

A.

Affiliation

Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Egypt & Department of Medical Laboratories and Medical Biotechnology, Faculty of Applied Medical sciences, Taif University, Turabuha branch, KSA.

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Soliman

MiddleName

M.

Affiliation

Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt.

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Nassan

MiddleName

A.

Affiliation

Department of Pathology, Faculty of Veterinary Medicine, Zagazig University,

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Mansour

MiddleName

A.

Affiliation

Department of Genetics, Faculty of Agriculture, Ain Shams University, Egypt.

Email

-

City

-

Orcid

-

Volume

5

Article Issue

2

Related Issue

6952

Issue Date

2012-10-01

Receive Date

2012-04-07

Publish Date

2012-10-01

Page Start

83

Page End

104

Print ISSN

1687-9988

Online ISSN

2357-0504

Link

https://jva.journals.ekb.eg/article_44876.html

Detail API

https://jva.journals.ekb.eg/service?article_code=44876

Order

7

Type

Original Article

Type Code

782

Publication Type

Journal

Publication Title

Journal of Veterinary Anatomy

Publication Link

https://jva.journals.ekb.eg/

MainTitle

Immunohistochemical, cellular localization and expression of inhibin hormone in the buffalo (Bubalus bubalis) adenohypophysis at different ages

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Article

Created At

22 Jan 2023