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42681

Some Comparative Immunohistochemical Studies on the Ductus Deferens in the Donkey (Equus asinus) and Water Buffalo Bull (Bubalus bubalis).

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Last updated: 22 Jan 2023

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Abstract

The current work aimed to apply immu- nohistochemistry (IHC) to evaluate the differential distribution pattern of some biologically active proteins in the ductus deferens (DD) in two different animal species common in the Egyptian farms. Immunolocalization of S100, angiotensin converting enzyme (ACE), α- smooth muscle actin (α-SMA), connexin 43 (Cx43) and galactosyltransferase (GalTase) was practiced on paraffin- embedded sections of DD from clinically healthy adult five donkeys and five water buffalo bulls. The DD, in both species, was lined by a folded, high cuboidal (in donkey) or tall columnar (in buffalo) pseudostratified epithelium with stereocilia. IHC revealed that all antibodies used, displayed variable reactivities in the two species. Nerves and the periductal smooth muscles expressed a strong immunoreactivity (IR) for S100and α-SMA respectively; both in the donkey and in the buffalo. Stereocilia of principal cells showed a marked ACE- immunostaining in the donkey but not in the buffalo. Cx43-binding sites were found between the basal portions of the ductal epithelium and the subjacent interstitium; as well as among the periductal smooth muscle cells (SMCs) only in the buffalo bulls. Distinct GalTase- binding sites were found in the ductal epithelium only in the donkey. In conclusion the present findings exhibited a species-specific pattern of distribution for most of proteins under study, sug- gesting a variable functional signific- ance of the DD in the different animal species.

DOI

10.21608/jva.2010.42681

Keywords

donkey, Ducts deferens, Immunohistochemistry, Water buffalo

Authors

First Name

Mohamed

Last Name

Alkafafy

MiddleName

-

Affiliation

Department of Cytology and Histology, Faculty of Veterinary Medicine, Minuofiya University, Sadat City Branch, Egypt

Email

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City

Sadat City

Orcid

-

First Name

Hossam

Last Name

Attia

MiddleName

-

Affiliation

Department of Histology and Cytology, Faculty of Veterinary Medicine, Benha University, Tokheh, Kaliobia, Egypt.

Email

-

City

Benha

Orcid

-

First Name

Reda

Last Name

Rashed

MiddleName

-

Affiliation

Department of Anatomy and Embryology, Faculty of veterinary Medicine, Minoufiya University, Sadat City branch, Egypt.

Email

-

City

Sadat City

Orcid

-

First Name

Mohamed

Last Name

Kandiel

MiddleName

-

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Tokheh, Kaliobia, Egypt.

Email

-

City

Benha

Orcid

-

Volume

3

Article Issue

1

Related Issue

6683

Issue Date

2010-04-01

Receive Date

2010-02-02

Publish Date

2010-04-01

Page Start

55

Page End

69

Print ISSN

1687-9988

Online ISSN

2357-0504

Article File

JVA_Volume 3_Issue 1_Pages 55-69.pdf

PDF . 912.9kB

Link

https://jva.journals.ekb.eg/article_42681.html

Detail API

https://jva.journals.ekb.eg/service?article_code=42681

Order

5

Type

Original Article

Type Code

782

Publication Type

Journal

Publication Title

Journal of Veterinary Anatomy

Publication Link

https://jva.journals.ekb.eg/

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Article

Created At

22 Jan 2023