Twenty-six buffalo fetuses ranging from 6.2- 103 cm CVRL (56 - 305 days-old) were used in this study. Twenty fetuses were used to study the eyelids mor- phogenesis and six fetuses were used to study the vasculogenesis and micro- vasculatures of the eyelids. The fused superior and inferior eyelids were rec- ognized at 6.2 cm (56 days). The fusion started to separate at 50 cm CVRL (186 day) while the eyelids appeared grossly opened at 60 cm CVRL (209 days). The palpebral skin and conjunctiva exhibit remarkable developmental changes throughout the gestation period. The primordia of the hair follicles and orbicu- laris oculi muscle were observed at 10.5 cm CVRL (76 days). The primordium of the tarsal glands was seen at 13 cm CVRL (87 days), while the primordia of the sweat and sebaceous glands were first demonstrated in fetus of 21 cm CVRL (121 days). Keratinization of the palpebral skin started at 38 cm CVRL (159 days) while the cornified skin with complete cutaneous structures and the papillae of tarsal glands openings were seen at 60 cm CVRL (209 days), thus it might be associated with eyelids reopening. The microvasculature of the eye-lids was started to differentiate into vas- cular networks at 19 cm CVRL (114 days), while the three cutaneous vascular networks, the perifollicular vascular networks of the cilia besides the mu- cosal and submucosal networks of the conjunctiva appeared fully developed at 80 cm CVRL (254 day). Finally one could conclude that the early differentiation and maturation of eyelids micro-vasculature might be associated with the early development of the eyelids structures.