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45535

Influence of soybean phytoestrogens exposure on the Mouse Stem cells differentiation

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Last updated: 04 Jan 2025

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Abstract

The toxic effects and mechanism of soy isoflavones such as genistein and diadzein on the early embryonic development and differentiation are still unknown. We aimed to elucidate the developmental and differentiation alterations occurred after either single exposure or co-exposure of soy isoflavones (Genistein and Di- adzein) on the mEScs in order to un- derstand the effects and possible pathways of phytoestrogen treatments on the early embryonic development. In the present study, mouse embryonic stem cells (mEBs) differentiate when exposed to differ- ent concentrations of genistein and/or diadzein for 5 days and their proliferation, apoptosis and differentiation capacities were evaluated using RT-qPCR analysis. Our results confirmed that soy phytoestrogens had differential effects on regulation of proliferation and apoptosis and the differentiation ability of the mouse stem cells at different levels varies from increase in the differentiation markers of the three germ layers or only ectoderm markers together with up regulation of Oct4 and Rex1 transcription factors and in some cases with down regulation of Nanog in comparison with the control condition and these variations depend on the concentration and whether single or co-treatment of these soy phytoestrogens were used. All of these effects appeared to be related to down regulation effect of Esrrb. Our entire result concluded that soy isoflavones might disturb differentiation of mEBs and these phytochemicals may function via the estrogen relatedreceptor β (ESRRβ)-mediated pathway.

DOI

10.21608/jva.2018.45535

Keywords

Phytoestrogens, Differ- entiation, Embryonic Stem cell, ESRRβ

Authors

First Name

T.

Last Name

El-Mahdy

MiddleName

O.M.

Affiliation

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.

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City

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Orcid

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First Name

S.

Last Name

El-Nahla,

MiddleName

M.M.

Affiliation

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.

Email

-

City

-

Orcid

-

First Name

S.

Last Name

Takahashi

MiddleName

-

Affiliation

Laboratory Animal Resource Center, University of Tsukuba, Tsukuba, Ibaraki, Japan & Department of Anatomy and Embryology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Basha

MiddleName

W.

Affiliation

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.

Email

walaaanatmy85@yahoo.com

City

Ismailia, Egypt.

Orcid

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Volume

11

Article Issue

2

Related Issue

6442

Issue Date

2018-10-01

Receive Date

2018-09-07

Publish Date

2018-10-06

Page Start

69

Page End

90

Print ISSN

1687-9988

Online ISSN

2357-0504

Article File

JVA_Volume 11_Issue 2_Pages 69-90.pdf

PDF . 1.8MB

Link

https://jva.journals.ekb.eg/article_45535.html

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https://jva.journals.ekb.eg/service?article_code=45535

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Original Article

Type Code

782

Publication Type

Journal

Publication Title

Journal of Veterinary Anatomy

Publication Link

https://jva.journals.ekb.eg/

MainTitle

Influence of soybean phytoestrogens exposure on the Mouse Stem cells differentiation

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Article

Created At

22 Jan 2023