In this study, we aim to evaluate the effect of stress from niddle injection and oral gavaging on gross anatom- ical parameters, histology, histo- chemistry of the testes as well as spermatogenesis and spermatozoa morphology in Wistar Rat. Twenty one male Wistar ratswere divided into three groups. Group A were not exposed to any stress, Group B rats were exposed to needle stress by intraperitoneal treatment with 1 ml/kg body weight of distilled water three times a week and group C rats were treated orally with 1 ml/kg body weight of distilled water three times a week. The protocol lasted for 8 weeks. There were no significant (p>0.05) difference in body weight, testis weight and volume, spermatozoa count, spermatozoa motility, spermatogonia, spermatocyte, spermatid, percentage number of morphologically normal sperma- tozoa and in the percentage number of sluggish, immotile and morphologically abnormal spermatozoa of rat exposed to stress. These rats showed normal histological and histochemical profiles. Also observed were normal geometric parameters across the groups. The present study has shown that stress from research does not affect the gross, histology, histochemistry of the tes- tes, spermatogenesis and spermatozoa morphology in Wistar Rat (Rattus norvegicus).